Just-a-Tube  Buccal Swab Genomic DNA Isolation Kit

The Just-a-Tube^?Buccal Swab Genomic DNA Isolation Kit is designed and optimized for easy and fast isolation of high-quality gDNA from buccal swab or brush samples. DNA sample purification can be processed just in a micro-tube in less than one hour. No silica membrane and magnetic beads are needed for DNA binding in the process. Based on Charm Biotech developed Solid Surface Reversible Binding (SSRB) technology, the Just-a-Tube system utilizes micro-tubes coated with proprietary turbo-binders acting to selectively capture and efficiently bind genomic DNA from buccal cell lysates. In the presence of Binding Buffer gDNA specifically interacts with the turbo binders and binds to the tubes while proteins and other contaminants will remain in the solution. Unbound material is removed in washing steps. The purified gDNA can easily be eluted in 10 mM Tris Elution Buffer or water. The purified ready-to-use gDNA is ideal for downstream application such as restriction digestion, primer extension, SNP analysis, PCR, sequencing, whole genome amplification (WGA) and DNA methylation analysis.

Feature Highlight

• Easy to handle: All procedures have been optimized with the Charm Basket (CB3)(patent pending) and Binding Tube (BT3) for ease-of-use. There is no sample transfer between different tubes during purification. Buccal swab sample digestion, collection, DNA binding and DNA storage all are processed in one combined basket tube. This makes the whole procedure very easy to perform.
• Reliable quality: Consistent tube-to-tube performance and unique solid-surface capture technology ensure the isolation of high-quality gDNA without contamination.
• Maximum recovery: This novel solid-surface capturing plateform not only allows fast buffer washing and easy sample elution, but also provides maximum sample capture and release from buccal cell lysates.

Kit Specifications:

• Starting Material:  Buccal Swab or Buccal Brush

• Elution Volume: 20 ul - 100 ul

• DNA Quality: OD 260/280  > 1.7
• DNA Yield:  In the range of 0.5 ug - 4 ug, mostly 1 -2 ug.

Please check similar product:  Just-a-Plate 96 Buccal Cell gDNA Isolation Kit

Order Information

Note: Individual kit components can be purchased separately, Please call for details.