Just-a-Plate ^™ 96 Genomic DNA Isolation Kit

The Just-a-Plate^™ 96 Genomic DNA Isolation Kit is designed and optimized for simple, fast and high-throughput isolation of gDNA from cultured mammalian cells and tissues. Up to 96 DNA samples can be simultaneously extracted in a 96-well microplate format in less than 50 minutes. No spin-column, filter plate, silica membrane or magnetic beads are needed for the isolation and no vacuum and filtration are required in the process.  Based on charm biotech developed Solid Surface Reversible Binding (SSRB) technology (patent pending), Just-a-plate system employs the solid 96-well plate surface coated with proprietary turbo-binders as selective nucleic acid capturers to efficiently extract gDNA from tissues and cells. DNA molecules can specifically interact with the turbo binder in the extraction buffer, while proteins and other contaminants are not bound and remain in the solution. After washing the plate with washing buffer to remove unbound material, the purified gDNA can be easily eluted in 10 mM Tris Elution Buffer or water. Without using vacuum, the unique centrifugation-based procedure of the Just-a-plate 96 Purification kit reduced opportunities for cross-contamination between wells. The plate used in the kit is compatible with a variety of automated liquid handler, centrifuges robots, pipetting devices, automated sequencers and PCR cyclers. The isolated ready-to-use gDNA is ideal for use in restriction enzyme digestion, SNP analysis, PCR, automated sequencing reactions, offering superior data quality and read length. (For bacterial genomic DNA Isolation information, please send your request to infor@charmbiotech.com)

Feature Highlight

• Fast to perform: It only takes 50 minutes of overall time to purify 96 cultured mammalian cell samples. It means less than 1 minute for each gDNA isolation. No tedious vacuum “ drying” process as being used for 96-well plates with membrane.
• Easy to handle:  All procedures have been optimized with standard 96-well plates for ease-of-use. Elimination of multiple steps of filtration in the sample binding and washing step associated with conventional membrane method makes whole procedure very easy to perform. All downstream assays can be performed in the same purification plate without sample elution.
• Reliable quality: Consistent well-to-well performance, reliable cross-contamination prevention procedure and unique solid-surface capture technology provide consistent yield at high DNA quality without any contamination.

Order Information

Note: Individual kit components can be purchased separately, Please call for details.