Just-a-Plate ^™ 96 FFPE Total RNA/MicroRNA Isolation Kit

The Just-a-Plate^™ 96 FFPE Total RNA/MicroRNA Isolation Kit is designed and optimized for easy, fast and high-throughput isolation of high-quality total RNA and microRNA from FFPE tissues. No silica membrane, filter plate, or magnetic beads are needed and no chemical de-paraffin steps are required in the process.  Based on charm biotech developed Solid Surface Reversible Binding (SSRB) technology (patent pending), Just-a-plate system employs the 96-well plate surface coated with proprietary turbo-binders as selective nucleic acid capturers to efficiently extract RNA and microRNA from FFPE sample tissues. RNA molecules can specifically interact with the turbo binder in the extraction buffer, while proteins and other contaminants are not bound and remain in the solution. After washing the plate with washing buffer to remove unbound material, the purified total RNA and microRNA can be easily eluted in 10 mM Tris Elution Buffer or water. The novel sample buffers and process in this kit can also efficiently remove nucleic acid chemical modifications introduced by formalin fixation step in tissues embedding process. The modification-removed RNA molecules are better substrates being ready for use in downstream applications, such as reverse transcription, microRNA detection, quantitative RT-PCR (qRT-PCR) and other gene expression analysis. Since no silica membrane and beads have been used in the plate, there are no dead spaces and sample trapping problems related with membrane or bead-based column or plate methods. These features not only allow fast buffer washing and easy sample elution, but also provide the maximum sample recovery, which is extremely important when the sample amount is limit and there is no room for any sample loss.

Feature Highlight

• Fast to perform: After tissue section, it only takes 10-15 minutes of hand-on time to purify 96 FFPE tissue samples. No tedious vacuum “ drying” process as being used for 96-well plates with membrane.
• Easy to handle: Both de-paraffin and total RNA isolation will be processed in the plates provided. No need to look for pellet in the regular chemical de-paraffin method. No vacuum or magnetic force needed.
• Reliable quality: Consistent well-to-well performance, reliable cross-contamination prevention procedure and unique formamide cross-linking removal chemistry provide consistent yield at high RNA quality.
• Maximum recovery: Novel efficient binding technology provides maximum sample capture and release from limited sample sources without dead space or sample trapping problems usually associated with the membrane and beads based plates or column.
• Environmental-friendly procedure: No chemical organic solvents used for de-wax and no chaotropic reagents, such as guanidinium used in the binding step as in typical silica matrix methods.

Order Information

Note: Individual kit components can be purchased separately, Please call for details.