How do you ensure optimal sample preparation, including RNA extraction, normalization , reverse transcription and  amplification in microarray analysis?

How do you validate your real-time PCR assays?

What controls and replicates do you use in your end-point and real-time PCR reactions?

How do you determine the efficiency of the real-time PCR assays?

How do you choose normalization genes for technical variations

What procedures do you take to ensure you have good labelling and hybridization probes in your CGH array?