Charm-Pure ^™ Genomic DNA Isolation Kit (Tissues and Cells)

The Charm-Pure^™ gDNA Isolation Kit (Tissues and Cells) is designed and optimized for easy and fast isolation of high-quality gDNA from tissues and cells. Charm-Pure system employs special turbo-binding membranes as selective nucleic acid captures in a familiar spin column format to efficiently extract gDNA from tissues and cells with higher yield and purity than conventional silica membrane. Genomic DNA molecules can specifically interact with the turbo-binding membrane in the extraction buffer, while proteins and other contaminants are not bound and remain in the solution and passed through. After washing the column with washing buffer to remove unbound material, the purified gDNA can be easily eluted in 10 mM Tris Elution Buffer or water and is ready for use in downstream applications, such as restriction enzyme digestion, primer extension, PCR, whole genome amplification (WGA) and other genomic analysis.

Feature Highlight

• Fast and easy to perform: Using easy-spin column in a streamlined protocol with less than 15 min hands-on time to isolate gDNA from tissues and cells.
• Higher yield  and better quality: Consistent turbo-binding membrane super capturing capacity provides high yield of gDNA with demonstrated improved downstream performance in applications such as PCR, enzyme digestion and southern blotting.
• Technical data: Process 10 to 25 mg tissues or 5 x 10⁶ mammalian cells and about  10-40 ug gDNA can be extracted based on the sample types. Purified gDNA is usually in the size range of 20-50 kb.

Order Information

Note: Individual kit components can be purchased separately, Please call for details.