Fragments such as promoter, enhancer, terminator, secretion
leader, IRES, N and C terminal tags, resistance genes, selection markers, and
fluorescence protein genes can all be cloned and modified for a shuttle or
Lambda based (attB, attP, attL and attR) custom
recombination entry and expression vectors can be constructed upon request.
This could include vectors capable of doing multiple fragments swaps and
Recombinase or ligase linked vector can be custom made
for high throughput cloning purpose. We use novel topoisomerase (Shope Fibroma
Isomerase) linked vector for directionally cloning of gene of interest.
Glycerol stocks or DNA
Annotated plasmid map and sequence file
DNA purification with resin based technology
Vector construction and modifications
Sequencing confirmation of constructs
Digestion of DNA to confirm identity of the constructs
DNA or glycerol stocks together with a detailed