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How do you ensure optimal sample preparation, including RNA extraction, normalization , reverse transcription and  amplification in microarray analysis?

 

How do you validate your real-time PCR assays?

 

What controls and replicates do you use in your end-point and real-time PCR reactions?

 

How do you determine the efficiency of the real-time PCR assays?

 

How do you choose normalization genes for technical variations

 

What procedures do you take to ensure you have good labelling and hybridization probes in your CGH array?

 

How do neucleotide composition affect UV260:280 ratio during nucleic acid UV spectrophotometer reading?

 

 

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