We have experience in cloning human and mouse
monoclonal antibodies for expression in mammalian cells. We use CMV driven
mammalian expression vectors for high-level heavy and light chain expression.
The benefit of the method eliminates the need for mouse sacrifice and animal
handling. It provides a high throughput production method using stably
integrated, antibody producing mammalian cells.
Monoclonal antibody producing hybridoma cells
Total RNA isolation
Heavy and light chain variable region cloning
Assembly full length heavy and light chain with secretion
leader sequence and constant region
Heavy and light chain cloning
Sequence confirmation of constructs
Digestion of plasmid DNA to confirm identity of the
Glycerol stocks for heavy and light chain expression
constructs together with a detailed report.