Lignocellulosic biomass is made up with about 50% cellulose,
25% hemicelluloses, and 25% lignin. Efficient degradation of biomass into
fermentable sugar is the key limiting factor in biofuel research and
application. Charm Biotech has in-depth knowledge in these key areas of biomass
degradation. We specialized in enzyme engineering, strain construction and
optimization. We welcome collaborations and business development as this is new
and evolving field. Our goal is to produce sugar economically by engineer
yeasts or other organisms so they can degrade biomass while fermenting sugars
We provide services in expression of different types of
lignocellulosic degrading enzymes from various fungal/yeast/bacterial sources.
We can do codon optimization; provide a selection of secretion signals and
terminators for proper expression and secretion. We can also integrate tested
expression cassettes into genomic loci for stable integration and expression.
1. Cellulose degradation enzymes
The Key cellulose enzymes in
cellulose degradation we provide:
Exo -1,4 Glucanases (cellobiohydrolases, CBHs)
Applications: Cellulases catalyze
the hydrolysis of cellulose. They can be used for commercial food processing in
coffee. It performs hydrolysis of cellulose during drying of beans.
Furthermore, cellulases are widely used in textile industry and in laundry
detergents. They have also been used in the pulp and paper industry for various
purposes, and they are even used for pharmaceutical applications.
2. Lignin degradation enzymes
The Key enzymes in lignin degradation
lignin-degrading enzymes are key enzymes needed for lignin remove and recycle
in nature. They can be used in pulp bleaching, waste water treatment, skin and
hair lightening etc.
3. Hemicellulose degrading enzymes
The Key enzymes in hemicelluloses
degradation we provide:
Applications: Xylanase and β-D-Xylosidase are enzymes that can degrade the linear
polysaccharide beta-1,4-xylan into its monomer xylose, thus breaking down
hemicellulose, one of the major components of plant cell walls. These enzymes
can be used for pulp bleaching in place of toxic chlorine compounds. It can
also be used in animal feeds to improve sugar content from xylan and used in
bakery to increase quality of bread.
4. Other enzymes/proteins we can provide for biomass
Ferrulic acid esterase
Applications: Ferulic acid esterase
is involved in hydrolysing recalcitrant parts of the plant cell wall, the
beginning of a process that enables more complete breakdown of fibrous or
pectinaceous cell walls and consequently the release of a higher proportion of
sugars or cell wall bound flavors. It can be used in fruit juice and vegetable
Applications: Lipase is an enzyme
that catalyzes the hydrolysis of ester chemical bonds in water-insoluble lipid
substrates. Lipases can be used in baking, laundry detergents and even as
biocatalysts to convert vegetable oil into fuel.
5. Enzymatic assay to determine activity
We have specific assays for each of
these enzymes to determine their activities in functional units.
6. Expression systems
We can express them in bacterial,
fungal, yeast (Saccharomyces cerevisiae), and Pichia expression systems. By adding secretion signal, we can
express and secret them in yeast and Pichia
culture for direct biomass conversion. We can also use native secretion signal of
particularly enzyme if desired.
7. Integration into yeast genome
We have specific loci that we can
integrate yeast genome for active expression of the target genes. These sites
are proven and tested for effective expression and secretion.
For more information and
price on expression of specific enzyme and strain construction, please call Charm
Biotech at 858 882 7701. We will provide services from design of project, gene
synthesis, codon optimization, cloning and expression, to activity assay and
delivery of integrated strain or expression clones with detailed project report.