The Just-a-Plate™ 96 Blood Genomic DNA
Isolation Kit is designed and optimized for easy, fast and high-throughput
isolation of gDNA from whole blood and buffy coat. No silica membrane, filter
plate, or magnetic beads are needed and no vacuum steps are required in the
process. Based on charm biotech developed Solid
Surface Reversible Binding (SSRB) technology (patent pending), Just-a-plate system employs the 96-well plate surface
coated with proprietary turbo-binders as selective nucleic acid capturers to
efficiently extract gDNA from 20-40 ul. blood. DNA can specifically interact
with the turbo binder in the extraction buffer, while proteins and other
contaminants are not bound and remain in the solution. After washing the plate with washing buffer to remove
unbound material, the purified gDNA can be easily eluted in 10 mM Tris Elution
Buffer or water. The purified gDNA
molecules are ready for use in downstream applications,
such as SNP detection, primer extension, PCR, whole genome
amplification (WGA) and other genomic DNA analysis.
Since no silica membrane and beads have been used in the plate, there are no
dead spaces and sample trapping problems related with membrane or bead-based
column or plate methods. These features not only allow fast buffer washing and
easy sample elution, but also provide the maximum sample recovery.
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