The Just-a-Plate™ 96 Total RNA Isolation Kit is designed and optimized for simple, fast and high-throughput
isolation of total RNA from cultured cells and tissues. Up to 96 RNA samples can
be simultaneously extracted in a 96-well microplate format in less than 30
minutes. No spin-column, filter plate, silica membrane or magnetic beads are
needed for the isolation and no vacuum and filtration are required in the
process. Based
on charm biotech developed Solid Surface Reversible Binding (SSRB)
technology, Just-a-plate system
employs the solid 96-well plate surface coated with proprietary turbo-binders
as selective nucleic acid capturers to efficiently extract RNA from
tissues and cells. RNA molecules can specifically interact with the turbo binder in the extraction
buffer, while proteins and other contaminants are not bound and remain in the
solution. After washing the plate with washing buffer to
remove unbound material, the purified RNA can be easily eluted in 10 mM
Tris Elution Buffer or water.The plate used in the kit is compatible with a variety of automated liquid
handler, centrifuges robots, pipetting devices, automated sequencers and PCR
cyclers. The isolated total RNA is ideal for use in gene expression analysis,such as RT and q RT-PCR.
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