The Charm-Pure™ Blood gDNA Isolation Kit is designed and optimized for easy and fast isolation of high-quality gDNA and viral DNA (e.g. HBV) from human whole blood, serum, plasma, or other body fluids.Charm-Pure system employs special turbo-binding membranes as selective nucleic acid captures in a spin
column format to efficiently extract gDNA from blood with higher yield and purity than conventional silica membrane. Genomic DNA molecules can specifically interact
with the turbo-binding membrane in the extraction buffer, while proteins and other
contaminants are not bound and remain in the solution and passed through. After washing the column with washing buffer to remove
unbound material, the purified gDNA can be easily eluted in 10 mM Tris Elution
Buffer or water. and be used directly in downstream applications,
such as restriction digestion, primer extension, PCR, whole genome
amplification (WGA) and other genomic analysis. To purify pure viral DNA without cellular DNA, we recommend usage of cell-free sample (serum or plasma) as viral DNA copurified with cellular DNA.