How Just-a-Plate 96 PCR Purification Kit Works

The Just-a-Plate^™ 96 PCR Purification Kit is designed and optimized for simple, fast and high-throughput purification of PCR products directly from the same PCR reaction plate. Up to 96 DNA samples can be simultaneously purified in a 96-well microplate format in less than 20 minutes. After PCR reaction, without transferring, PCR products can be purified in the same PCR reaction plate with high efficiency. No spin-column, filter plate, silica membrane or magnetic beads are needed for the isolation and no vacuum and filtration are required in the process.  Based on Charm Biotech developed Solid Surface Reversible Binding (SSRB) technology, Just-a-plate system employs the solid 96-well plate surface coated with proprietary turbo-binders as selective nucleic acid capturers to efficiently bind PCR DNA from reaction mixtures. DNA molecules can specifically interact with the turbo binder in the extraction buffer, while primers, nucleotides and other contaminants are not bound and remain in the solution. After washing the plate with washing buffer to remove unbound material, the purified PCR prodcuts can be easily eluted in 10 mM Tris Elution Buffer or water. Without using vacuum, the unique centrifugation-based procedure of the Just-a-plate 96 Purification kit reduced opportunities for cross-contamination between wells. The plate used in the kit is compatible with a variety of automated liquid handler, centrifuges, robots, pipetting devices, automated sequencers and PCR cyclers. The isolated ready-to-use PCR products are ideal for use in restriction enzyme digestion, SNP analysis, automated sequencing reactions, offering superior data quality and read length.