The Charm-Pure™ Virus Nucleic Acid Isolation Kit is designed and optimized for easy and fast isolation of high-quality vial DNA and RNA from cell-free biological fluids such as plasma,
serum, saliva, sputum and CSF (cerebrospinal fluid) as well as cell culture supernatant. Charm-Pure system employs special turbo-binding membranes as selective nucleic acid captures in a conventional spin-column format to efficiently extract vial nucleic acid from body fluid samples with higher yield and purity than conventional silica membrane. Vial DNA and RNA molecules can specifically interact
with the turbo-binding membrane in the extraction buffer, while proteins and other
contaminants are not bound and remain in the solution and passed through. After washing the column with washing buffer to remove
unbound material, the purified gDNA can be easily eluted in 10 mM Tris Elution
Buffer or water. The isolated viral nucleic acids is suitable for vial detection, genotyping and quantification.